Application

Pulmonary function, gene expression, healing & repair process, differentiation, extracellular matrix production, growth factor and cytokine activation, actin reorganization, synthesis of type II collagen, elastin, and proteoglycans, airway remodeling, pharmaceutical development.

Cell Line Origin

Lungs

Components

Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO

General description

RLF from Cell Applications, Inc. have been used to demonstrate that exaggerated (compared to adults) NF-κB activation in neonates in response to hyperoxia is mediated by phosphorylation of IκBα on Tyr42 (Wright, 2009). They were also used to elucidate melatonin effects and signaling pathway (Ben Soussia, 2012) and served as a control in studies investigating epithelial-mesenchimal transition as the source of myofibroblasts responsible for idiopathic pulmonary fibrosis (Willis, 2005; Jain, 2007).

Preparation Note

2nd passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO Can be cultured at least 10 population doublings

Quality

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

Subculture Routine

Please refer to the RLF Culture Protocol.